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Currently, generally recognized is the multiple hit theory, which uses insulin resistance (IR) and obesity the first hit to increase free fatty acids (FFAs) and make the liver more susceptible to hepatotoxic injury

This stock solution was diluted with PBS so that 200 L of the sample had an initial absorbance of 1.0 at 734 nm in a well of a 96-well microplate

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Materials and methods Study design and participants This single-center, prospective, case-control study was conducted between May 1, 2024, and September 1, 2024, at the Rheumatology Outpatient Clinic of Recep Tayyip Erdoan University Training and Research Hospital