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Slides were mounted with Fluoromount-G (Southern Biotech, USA) and examined using a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems, Germany) equipped with HCX PL APO 63 oil (NA = 1.4) objectives

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After incubation, cells were rinsed with saline and incubated with 100 mM CuSO 4 (Sigma-Aldrich, C1297) for 2 min

Cell death mediated by the pyroptosis pathway with the aid of nanotechnology: prospects for cancer therapy