Mitochondrial DNA promotes nlrp3 inflammasome activation and contributes to endothelial dysfunction and inflammation in type 1 diabetes
(PubMed) Mathew MC, Ervin AM, Tao J, Davis RM
In conclusion, treatment with DPP-IV inhibitor resulted in a greater reduction in HbA1c in subjects with high GLP-1 compared to low GLP-1 levels
Methods Peptide agonists and general reagents Rat amylin (rAmy), salmon calcitonin (sCT) and human calcitonin (hCT) were purchased from China Peptides
Antibodies The following rabbit polyclonal antibodies were used: anti-GSTM2 (#ab125,102, Abcam, Cambridge, UK, 1:1000), anti-GSTM2 (#A13,496, ABclonal, Wuhan, China, 1:2000), anti-GAPDH (#CSB-PA00025A0Rb Flarebio Biotech LLC, College Park, MD, USA, 1:1000), anti-CIDEC (#D222114, Sangon Biotech, Shanghai, China, 1:1000), anti-CIDEC (#12287-1-AP, Proteintech, Rocky Hill, NJ, USA, 1:1000), anti-ADRP/perilipin2 (#15294-1-AP, Proteintech), anti-DGAT2 (#bs-12,998R, BIOSS, Beijing, China), anti-DGAT2 (#A13,891, ABclonal, Wuhan, China, 1:2000), anti-GPAT4 (#bs-15,587R, BIOSS), anti-SREBP1 (#14,088-1-AP, Proteintech), and anti-PCYT1A (#bs-11306R, BIOSS), anti-PCYT1A (#A5935, ABclonal, Wuhan, China, 1:1000)
The fluidity of NCBSO solution was still good after incubating with GSH for 24 h