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They were cultured, respectively in Dulbecco's Modified Eagle Medium, Iscove's Modified Dulbecco's Medium, and Roswell Park Memorial Institute 1640 medium (Hyclone) with 10% fetal bovine serum (Gibco) under incubation at 37C with 5% carbon dioxide

Nat Rev Endocrinol 6:279289 Keenan HA, Sun JK, Levine J, Doria A, Aiello LP, Eisenbarth G, Bonner-Weir S, King GL (2010) Residual insulin production and pancreatic -cell turnover after 50 years of diabetes: Joslin Medalist Study

However, it does not appear to directly lead to an increase in fasting blood glucose

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