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The samples were centrifuged at 5000 RPM for 7 minutes and 80 ,^ were transferred into previously prepared tubes containing 240 aL ice-cold acetonitrile

After 24 h, the transfected cells were seeded onto 384-well microtiter plates (3000 cells per well) in HBSS supplemented with 5 mM HEPES, 0.1% (w/v) bovine serum albumin (BSA) and 0.5 mM 3-isobutyl-1- methylxanthine, and stimulated with increasing concentrations of GLP-2 for 30 min at RT

physiology Heart circulatory physiology 295 (5), H2113H2127

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Currently, there is no official guidance from NICE or the MHRA regarding the combined use of these agents