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Primary antibodies targeting specific proteins (Table S1) were incubated with membranes overnight at 4 C on a shaker

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Current-voltage (IV) relationships were measured by applying voltage steps with a Digidata 1550B with Humsilencer (Axon Instruments) controlled by an IBM-compatible computer with Axon TM pClamp11 TM software (Molecular Devices)

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, 42 , 2105-2117, doi: Drayton, R

The 24-week mark in the Phase 2 trial showed the 12 mg group had lost an average of 17.5% of body weight