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HEK293T cells (Cell Bank of Chinese Academy of Sciences, Shanghai, China) and human immortalized HSC cell line LX-2 (Procell Life Science & Technology, Wuhan, China) were cultured in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin and streptomycin (Gibco, USA) at a humidified 37 C incubator with 5% CO 2

Additionally, SIRT3 deacetylates ceramide synthases, promoting ceramide accumulation and inhibiting complex III activity 184

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According to the researchers, the findings indicated that the two drugs may have neuroprotective effects for people without diabetes and may help slow the rate of cognitive decline in Alzheimers patients.