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DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted

Durante el tamizado de la composicin, se busca llevar a cabo la reduccin y estandarizacin del tamao de partcula (conminucion) por medio de mallas de acero inoxidable para producir polvos finamente divididos con tamao de partcula controlado

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R., Wouters, H