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As a histone deacetylase (HDAC) inhibitor, butyrate alters gene expression in intestinal epithelial cells, promotes the synthesis of tight junction proteins, strengthens the intestinal barrier, and reduces the translocation of gut-derived toxins into the bloodstream, thereby indirectly protecting the central nervous system

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In brief, cultured differentiating chondrocytes were incubated with 50 M TPE-MI for 30 min at 37 C and collected for flow cytometry