To determine the optimal NaCl concentration to use as a diluent buffer for the triolein-substrate assay, we diluted PHP taken from four typical healthy subjects in KRP buffer containing 0.010.4 M NaCl and measured LPL activity using the triolein substrate assay described in Radiometric Detection of Lipoprotein Lipase Activity Using Triolein, ApoC-II, and Angiopoietin-Like 4. We found that LPL activity was highest when PHP was diluted in 0.175 M NaCl (Figure 3A), and therefore we recommend using 0.175 M NaCl when measuring LPL activity
JAMA Pediatr 172(10):e182293e182293 Popper SJ, Watson VE, Shimizu C, Kanegaye JT, Burns JC, Relman DA (2009) Gene transcript abundance profiles distinguish Kawasaki disease from adenovirus infection
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