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Evaluation by different mechanisms of the protective effects of vitamin B12 on methotrexate nephrotoxicity

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The principal factors of apoptosis program include Apaf-1 (apoptotic protease-activating factor 1), Bcl-2 (B cell lymphoma-2) family proteins and caspases (cysteinyl aspartate-specific proteinases) (Qin et al., 1999)

One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

Endocr Relat Cancer 2015;22:R183-204

47 Molecular biophysics also involves understanding the role of proteoglycans and other extracellular matrix components in maintaining the structural integrity and function of the ACL