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As noted by Foyer and Halliwell (1976), the chemical reaction between DHA and GSH is much more rapid at higher values of pH (about tenfold faster at pH 8 than pH 7

The higher dose in the second half of the cycle is designed to reinforce the collagen synthesis, sirtuin activation, and anti-inflammatory pathways initiated in Phase 1

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Thus, DNTB method grants measurement of free GSH, oxidized GSH (GSSG) and total content of GSH (free GSH + GSSG) in a multi-step approach in biological samples

This multi-pronged approach to tissue repair makes it valuable for conditions like eczema where damage occurs across multiple tissue layers and through multiple mechanisms