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Step-by-step injection technique: Remove the pen from refrigeration before injection to allow it to reach room temperature (refer to the IFU for specific timing recommendations) Wash hands thoroughly with soap and water Clean the injection site with an alcohol swab and allow to air dry completely Remove the pen cap and inspect the medication (should be clear and colorless) Place the clear base flat against the skin at a 90-degree angle Unlock the pen and press the injection button Hold the pen in place until you hear or feel a second click and the indicator shows the dose is complete (typically 10 seconds as specified in the IFU) Remove the pen from the skin and dispose of the entire pen in an FDA-cleared sharps container [1] Important notes: Do not inject through clothing

Serum from mice immunised with recombinant truncated PE demonstrated a strong bactericidal effect against NTHi (Ysebaert et al., 2019)