doi: 10.1016/J.AJME.2017.09.001 257 SinghYPPatelRNSinghYButcherRJVishakarmaPKSinghRKB
Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Standard Curve This product is detected by QC department and meets performance required in the manual
The rotational speed was then gradually increased to 40 rpm over a 5-minute period
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(A,C) Immunofluorescence staining was performed on cochlear frozen cryosection to determine the expression of GPX2 and GPX4 in P3 C57BL/6 mouse cochlea
This study demonstrated for the first time that disturbed NAD+ metabolism accelerates follicular atresia and ovarian reserve depletion by disrupting the microenvironment of oocytesomatic cell interactions, ultimately leading to a reproductive ageing phenotype [82]
