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IX-71 inverted microscope the pipette-to -bath DC resistance of patch electrodes ranged from 4.0 to 5.2 , and were filled with a internal solution of the following composition (in mM): 25 CsCl, 100 CSCH3O3S, 10 phosphocreatine, 0.4 EGTA, 10 HEPES, 2 MgCl 2) 0.4 Mg-ATP, and 0.04 Na-GTP

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GSTrap FF affinity columns are 1 ml or 5 ml prepacked columns that also are available and may be connected in series for scale-up