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Transfections of siRNA were carried out using Lipofectamine RNAi-Max (Life Technologies) following the manufacturers protocol

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For apoptosis assay, cells were suspended in Annexin V binding buffer (BD Science, New Jersey, USA) containing Annexin V-FITC (MBL International, Woburn, MA, USA) and propidium iodide (BD Science) for 15 min in dark

Obesity and Current Treatment Approaches: A Comprehensive Review