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It directly promotes glucose transport to small intestinal epithelial cells via sodium-dependent glucose transporter 1 through down-regulation of Ca 2+ and cAMP signaling pathways (46) and leads to increased energy expenditure by promoting uncoupling protein 1 gene expression in brown adipocytes through the cAMP-PKA signaling pathway (47)

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After that, the total amount of dopachrome produced in the enzyme reaction mixture was determined by absorbance at 490 nm by a microplate reader (Infinite F200, TECAN, Mnnedorf, Switzerland)

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