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After 24 h, cell viability was determined by MTS assay according to the instructions of the CellTiter 96 AQ ueous One Solution Cell Proliferation Assay System (Promega)

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Remember, miosis may not be present with co-ingestants or in cases of severe hypoxia

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However, as no consensus palmitoylation sequence motifs have yet been identified, the mechanism of zDHHC-PAT substrate specificity is only poorly understood at present (52)

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