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The inverse correlations identified in this study between PON-1 and inflammatory markers such as ADA and NLR further support the interconnectedness of oxidative stress and inflammation in RPL pathogenesis

RNA was isolated from liver and cell samples using TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, United States) and converted to cDNA using a reverse transcription kit from Thermo Fisher Scientific

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GSH assay kit was purchased from Beyotime Biotechnology (Nantong, Jiangsu, China)

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