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The sampling rate was set at 5 kHz and with a high-pass filter at 2 kHz .The recording pipettes (68 M) were filled with internal solution containing (in mmol/L) CsCl 120, CaCl 2 1, MgCl 2 2, EGTA 10, HEPES 10, ATP-Mg 2, GTP-Na 0.4, NaCl 5, and phosphocreatine 10

Cells were then washed, and dishes mounted on an inverted fluorescence microscope (Olympus IX71, Southall, UK) with a 40 oil-immersion objective

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