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GBM cells were cultured at 37 C and 5% CO 2 in Neurobasal medium (ThermoFisher) supplemented with 1x B27 without vitamin A (Invitrogen), 10 ng/ml recombinant human EGF (R&D systems), 10 ng/ml basic FGF (Bio-Connect), 1x L-glutamine (VWR), indicated further as complete NB medium [43, 44]

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5 Recent literature, such as a review published in JAMA in May 2024, put the data from the WHI study into context by indicating that the study was primarily in women in late menopause (over the age of 60) and focused on HRT for chronic diseases such as cardiovascular disease or dementia rather than its utility for vasomotor symptoms

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