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Amplification was performed in duplicate with the ABI 7300 system (Applied Biosystems) with the following profile: 50 for 2 min, 95 for 10 min, and 40 cycles of 95 for 15 s, and 60 for 1 min

Pain Reduction Begins Noticeable decrease in pain intensity, often 20-40% reduction

pAKT-Ser473, AKT, PPAR, PGC1, CPT1, Nrf2, UCP1 and pP38 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA)

Recruitment of GLUT-4 glucose transporters by insulin in diabetic rat skeletal muscle

This enables clinicians to make more informed decisions, improving treatment efficacy and reducing adverse effects