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Luminescence was recorded for 45 min at 1 min intervals using a Fluoroskan Ascent FL (Thermo Scientific, Rockford, IL, USA) fluorimeter (excitation: 538 nm, emission: 604 nm), and the slopes were used for calculations

It can compromise the integrity of painstakingly synthesized peptides, introduce variables you can't account for, and ultimately render your data useless

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Approximately 90% of the drug is rapidly inactivated by plasma pseudocholinesterase , while only about 10% reaches the neuromuscular junction (NMJ)