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SNAP/FLAG-hGLP-1R plasma membrane recycling INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto poly-L-lysine-coated black, clear bottom 96-well plates in full media, stimulated with vehicle or 100 nM exendin-4 for 1 hour at 37C to enable receptor internalisation, washed with PBS and incubated in 100 nM tetramethylrhodamine (TMR)-tagged exendin-4 (Ex-4-TMR) in full media for 1 hour or 3 hours at 37C to label any receptors that recycle back to the plasma membrane after agonist-mediated internalisation
PMID: 31305971 [18] Lisco G, De Tullio A, Disoteo O, et al
GLP-1RAs stimulate insulin secretion and suppress glucagon secretion, which may modify cardiac energy metabolism in T2DM patients [103]
A: BP-grade bacteriostatic water is the most widely referenced solvent in published protocols
These approaches systematically locate underutilized or optimal local interaction regions, simulating the dynamic and transient nature of PPIs, thereby presenting unlimited possibilities for efficient PPI drug discovery (Fig
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