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The cell pellets were washed with PBS and alkylated for 15 min at room temperature (RT) under denaturing conditions in 200 l of UCE-IAM buffer, consisting of 8 M urea, 1% (w/v) CHAPS, 1 mM EDTA, 200 mM TrisHCl pH 8.0 and 100 mM IAM as described (Rossius et al., 2018)

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