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Many promising biomarkers identified in small studies fail to replicate in larger validation cohorts

This measures the percentage of the target peptide peak relative to other detectable peaks under specific analytical conditions

Twelve samples preserved in 4% paraformaldehyde undergo ethanol dehydration through graded concentrations (85% for 2 h, 95% for 2 h, followed by 100% for 1 h each) before being cleared in xylene and embedded in paraffin