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By middle age, production has dropped significantly

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In mice with KRAS-driven pancreatic ductal adenocarcinoma (PDAC), high iron diets and GPX4 depletion, which results in 8-OHG release, lead to macrophage infiltration and activation (Dai E

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker