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The supernatant was collected to determine tissue ROS (E004-1-1), malondialdehyde (MDA, A003-1-2), protein carbonyls (PC, A087-1-2) and GSH (A006-2-1)contents, as well as tissue total antioxidant capacity (T-AOC, A015-1-2) and glutamylcysteine ligase (GCL, A120-1-1), superoxide dismutase (SOD, A001-1-2), glutathione peroxidase (GSH-Px, A005-1-2), catalase (CAT, A007-1-1), glutathione sulfotransferase (GST, A004-1-1) and glutathione reductase (GR, A062-1-1) activities