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All proteins in the collection are expressed as N-terminal GST-His6 fusion proteins in Saccharomyces cerevisiae, purified via glutathione-agarose beads in a high-throughput 96-well format, Sanger sequence-verified, and printed in duplicate onto nitrocellulose-coated glass slides as previously described [Venkataraman et al., 2018]

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In healthy people with normal B levels, additional B has no effect on energy metabolism