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Protein amounts were adjusted with the Pierce bicinchoninic acid (BCA) protein kit (ThermoFischer Scientific), and western blot analysis was performed using -Histidine antibody (Bethyl Laboratories Inc.)

Early nano-delivery systems such as liposomes and nanosuspensions initially suffered from storage instability (e.g., payload leakage and particle aggregation), but subsequent formulation optimization has greatly improved their robustness and yielded storage-stable products at scale (187, 188)

The 13 C-NMR measurements together with the infusion of 13 C-labeled substrates in mice (60), rats (163) and human (166) have shown that neuronal mitochondrial TCA cycle in the cerebral cortex contributes ~7085% of the total energy produced and the remaining (~1530%) by astroglia

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Troubleshooting Common Reconstitution Issues Even with a perfect protocol, problems arise

Prepared under controlled conditions, free of microbial contamination and endotoxins