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Immunohistochemical (IHC) methods were used to evaluate the expressions of acyl-CoA synthetase long-chain family member 4 (ACSL4) and glutathione peroxidase (GPX4) in the kidney tissues, reverse transcription-quantitative PCR and western blotting were used to assess the levels of ferroptosis marker factors in the kidney tissues and HK-2 cells, and flow cytometry was used to assess cell death
[5] [6] Other changes you might notice include increased hunger, faster return of appetite after meals, and potential changes in cardiovascular risk factors
10.1007/s00726-006-0477-9 Amino Acids 26 BlankeE
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