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4.6 Analytical advances revealing metabolic disruptions The rapid evolution of analytical technologies, including mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, has accelerated metabolomics-based DKD research (Zhang et al., 2025c)

In topical application, studies use concentrations of 0.01-1% in cream or serum formulations (Pickart et al., BioMed Res Int 2015For subcutaneous injection (research), doses vary depending on the experimental model

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