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Briefly, cells were suspended in sucrose-mannitol buffer (20 mM HEPES, pH 7.5, 2 mM EDTA, 70 mM sucrose, 220 mM mannitol, 5 mM NaF, protease inhibitor cocktail [Roche]) and homogenized using a Teflon homogenizer

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Similarly, the use of dupilumab in atopic eczema needs to be closely monitored for any anti- asthma effect in real life

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3.1.6 Piperine Piperine (1-peperoyl piperidine) is a simple and pungent piperidine-type alkaloid found in the seeds of black pepper ( Piper nigrum L