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As you can see on the cartoon above, first GST-X fusion is expressed in large amount (for example 250 ml to 1 l culture) under previously optimised conditions (for protein expression see post GGS LIVE - Protein expression in E.coli )

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This advanced peptide compound consists of 83 amino acids, featuring a 13 amino acid N-terminal extension at the N-terminus and an arginine substitution at position 3, changes that fundamentally alter its natural properties compared to native IGF-1

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Another variant of GIP is the C-terminally truncated form, GIP(1-30)NH 2

A plotter would have shown all of this in advance