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The mechanism appears to involve increased serotonin synthesis in substantia nigra, a regional pattern unlike any existing antidepressant drug

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Vitamin C and copper peptides should generally not be applied in the same routine: the low pH of L-ascorbic acid can destabilise GHK-Cu, and the combination has been linked to increased irritation in some users

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However, long-term data on maintenance without continued cycling remains limited, and individual responses vary