While kinase activity was generally conserved across drug treatments, the magnitude (fold enrichment compared to vehicle) and trend of substrate enrichment varied greatly (Fig
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Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis
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