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Even a small amount of bacteria can grow over time and ruin your valuable research compounds

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Based on the displayed structures, and in particular with that of the glutathione conjugate of etacrynic acid [132], which is itself a Michael acceptor, it is well conceivable that glutathione acrylate can dock into the glutathione activating pocket of the GST enzymes and irreversibly bind to the essential tyrosine phenolate function, thus inactivating the enzyme

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The homogenates were centrifuged twice at 98,560 g for 40 min at 4 C and the resulting supernatants were collected