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GPX4 [including cytosol- and mitochondria-localized GPX4 (GPX4 cyto and GPX4 mito )] is the first reported anti-ferroptosis pathway [55]

Mitochondrial thiols were detected by measuring the MFI of MBB in CD19 Tom20 + (Abcam ab186734) isolated mitochondria from the enriched FoB and MZB fractions

The workflow for this assay can be visualized as follows: This protocol refines the traditional DTNB method, removing the need for protein precipitation and strong acid treatment

This is due to a lack of regulation in their manufacturing process

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