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2.9.4 Interpreting the SYBR Green real-time PCR findings The Stratagene MX3005P program was used to determine each amplification curve and CT value using the deltadelta CT method described by 2.10 Analysis of liver histology Following the experiment, liver tissue samples were extracted from each group, cleansed, dehydrated, preserved in 10% neutral-buffered formalin, and embedded in paraffin