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HCT-116 colon cancer cell line from the NCI-60 Human Tumor Cell Lines (National Cancer Institute, USA), were grown in RPMI medium, supplemented with 10% fetal calf serum, 1% penicillinstreptomycin, and 1% glutamine

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It induces angiogenesis, enhancing endothelial cell migration and tube formation to recapitulate nutrient and oxygen delivery