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Common biomarkers used to identify senescent cells include the expression of p16 INK4a and p21 Cip1 , increased activity of SA--gal at pH 6.0, loss of the nuclear envelope protein Lamin B1, and the presence of persistent DNA damage foci [54]
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Arabidopsis mesophyll protoplasts were isolated from the leaves of the wild-type Col-0 ecotype, following a previously reported method (Yoo et al, 2007)