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low voltage electrophoresis was used for the upper layer of the gel, and the voltage was adjusted to 80V by turning on the power supply, and high voltage electrophoresis was used when the bromophenol blue entered the lower layer of the gel, and the electrophoresis was stopped when the voltage was adjusted to 120V until the bromophenol blue reached near the bottom of the gel

doi:10.1016/j.bbagen.2012.09.018

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