Prior to any characterizations, we ultrasonicated the nanoparticles for an hour
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In addition, GLP-1 also reduces ghrelin activity, a hormone with hunger-promoting effects [90, 92], and increases leptin sensitivity, a hormone with satiety-promoting affects [90, 93]
Finally, the membrane was incubated in SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific Pierce Protein Biology) and imaged with ChemiDocTMTouch Imaging system (BioRad)
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