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The supernatant was diluted to 1:50 in ice-cold 100 mM sodium phosphate buffer (pH 8.0), and NAD + was measured by an enzymatic cycling assay modified from the protocol of Graeff and Lee 40

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Consequently, riboflavin may significantly enhance cell longevity by boosting the activity and concentration of antioxidant enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase, that act on deactivating peroxides, which may create an oxidant environment (10, 43)

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