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This removes any ambiguity about when the medication needs to be replaced

After about the third application the pain in my hips lessened to the point that sitting down and getting back up wasn't nearly as bad as it had been

After adding 0.4 ml of 1 M sodium citrate buffer, pH 6.0, the mixtures were incubated at 37 C for 10 min with shaking at 125 rpm on a rotary shaker NR-3 (TAITEC) to facilitate release of bound sulfur as H 2 S gas from the aqueous phase

In addition, test buffers attached to commercial kits (BioVision, Mountain View, CA, USA) were used to disintegrate yeast cells in a mill, depending on the subsequent analysis of various antioxidative enzymes