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Chen Y, Swanson RA

Most researchers use the 1mL (100 unit) option

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Fewer side effects, or less severe side effects, or both, result from use of the described method than would result from administration of a therapeutically effective amount of a single agent sufficient to initiate the desired pharmacological response in the patient

This approach, which often involves applying a fraction of the standard dose, is designed to investigate the cellular or physiological responses without provoking the full-blown effects of the drug