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Insulin in the brain: sources, localization and functions

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Ghisari, M., Long, M., Tabbo, A

A minority of patients experienced an improvement in vision, and significantly decreased the extent of skin necrosis [26]