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Dalton M, Buckland N, Blundell J

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The full-length PGRMC1 cDNA fragment containing resistant sequences for shRNA was generated by using the primers (Supplementary Methods), and ligated into the Eco RI and Bam HI sites of the C-terminus of the 3xFLAG-tagged expression vector p3xFLAG CMV14 (Sigma)

This has not allowed me to do more with a leak, but has given me a much-needed reprieve in the onslaught of pain and meant I can tolerate a little more uptime

It also doesnt hurt that theyre loaded with vitamin C (great for cold and flu season), and are a good source of antioxidants, minerals, and fiber