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12.5 l of Dream Taq Green PCR Master Mix 2X (Thermo Scientific K1081), 1 l of primer 10 pmol (Metabion, Germany), and 1 l of template DNA (50 ng/L) made up the PCR reaction mixtures

The cells were cultured in DMEM (Gibco, Gaithersburg, USA) supplemented with 10% fetal bovine serum (Gibco, Gaithersburg, USA), 100 U/mL penicillin, and 100 g/mL streptomycin

These three studies represent thousands of data points across performance horses and pathology, offering unparalleled insight into safety, efficacy, and long-term function

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