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Plasma free fatty acid (FFA) concentrations were measured using the Free Fatty Acid Quantification Kit (Sigma-Aldrich) in separate cohorts of mice, including both sexes of whole-body Gpr146/ and Gpr146/ mice on HFD, as well as adipose-specific and liver-specific knockout models following HFD feeding

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The skin's inherent protective layer, specifically the outermost layer of the epidermis, stratum corneum, is a barrier that limits the entry of large hydrophilic molecules into the skin [27]